Figure 5

Glutamate-mediated cytosolic Ca²⁺ response is reduced in primary cortical neurons in the presence of the Ca²⁺ chelator BAPTA. Changes in cytosolic Ca²⁺ in Fluo-4 loaded neurons obtained by stimulation with 100 μM glutamate after 1 h of BAPTA treatment (cytosolic calcium chelator tetraacetoxymethyl ester, 50 μM) in ambient (A, E) or in 5% O₂ (B, F) conditions using 15 mM glucose (A–D) or 5 mM BHB (E–H) as solo substrate. Recordings from individual cells (gray and light red) and average (black and red) are shown from 4 independent experiments with a total of 183–380 cells. Quantification of the cytosolic calcium peak (C, G) and the area under the curve (D, H) upon glutamate stimulation was determined in ambient (black bars) and in 5% O₂ (empty bars) after 1 h of BAPTA treatment when indicated.