Figure 1
Larger postnatal brain volume in Tg21 mice. (A) Representative bright field sagittal brain images of hematoxylin and eosin staining (H&E) label in WT and Tg21 mice at postnatal ages (P): 3, 7, and 14. Scale bar: 1 mm. (B) Body weight across development in WT and Tg21 mice. Data are given as mean ± SD, WT (NP1-2 = 10, NP3 = 15, NP7 = 12, NP11 = 18, NP14 = 56, NP21 = 37, NP25 = 17 and NP60 = 15) and Tg21 (NP1-2 = 10, NP3 = 15, NP7 = 8, NP11 = 26, NP14 = 44, NP21 = 25, NP25 = 14 and NP60 = 27). Tg21 mice are slightly lighter after P21. 2-way ANOVA, F(1,333) = 5.6, P = 0.023. (C) Brain wet weight across development in WT and Tg21 mice. Data are given as mean ± SD, N equal as B. Greater brain weight is observed in Tg21 mice during postnatal development. 2-way ANOVA, F(1,333) = 178, P < 0.0001. Multiple comparisons: **P < 0.01, ****P < 0.0001. (D) Areas (labeled in Lila) in which the total volume analysis was made by stereology. (E–G) Volumes of brain areas across development in WT and Tg21 mice showing larger volumes in Tg21. N = 4 animals per age and genotype. (E) Brain volumes at P3. 2-way ANOVA, F(1,24) = 37.95, P < 0.001. Multiple comparisons: *P = 0.047, ****P < 0.0001. (F) Brain volumes at P7. 2-way ANOVA, F(1,24) = 41.01, P < 0.001. Multiple comparisons: *P = 0.027, ****P < 0.0001. G) Brain volumes at P14. 2-way ANOVA, F(1,24) = 50.8, P < 0.001. Multiple comparisons: ***P = 0.0002, ****P < 0.0001.
