Figure 8
EPO stimulates motoric development and motor coordination learning. Physical development in: (A) WT and Tg21 mice, and (B) Sox10-cre;EpoRfx/fx mice, showing the postnatal age at which skin pigmentation, fur appearance, incisor eruption, and eye-opening occurred. N = 10 animals per genotype. Appearance of reflexes in: (C) WT and Tg21 mice, and (D) Sox10-cre;E10-poRfx/fx mice. Shown are righting reflexes, cliff avoidance, negative geotaxis, and vestibular drop. N = 10 animals per genotype. Motoric development in: (E) WT and Tg21 mice, N = 15 animals per genotype; 2-way ANOVA, F(2,56) = 3.18, *P = 0.048, and (F) Sox10-cre;EpoRfx/fx mice. N = 10 animals per genotype. Shown are the ages at which pups started pivoting, crawling, and walking. Motoric open field test across postnatal ages P8 to P14 in: (G) WT and Tg21 mice. 2-way ANOVA, F(1,169) = 30.98, ****P < 0.0001). Multiple comparisons: *P = 0.0134, **P = 0.0015, ****P < 0.0001, N = 12–20 animals per genotype and age, and (H) Sox10-cre;EpoRfx/fx mice. N = 6–10 animals per genotype and age. (I–K) Rotarod motor coordination tests across postnatal ages in WT and Tg21 mice. (I) Shown are the average values from the four trials per animal at P14, P21 and P60. 2-way ANOVA, F(1,114) = 27.48, ****P < 0.0001. Multiple comparison: **P = 0.0051, ****P < 0.0001. N = 20 animals per genotype and age. (J) Rotarod motor coordination tests in WT and Tg21 mice at P14. Shown are all four trials per test. 2-way ANOVA, F(1,152) = 35.36, P < 0.0001. Multiple comparisons: **P = 0.0093, ***P = 0.0006. N = 20 animals per genotype (K) Rotarod motor coordination tests in WT and Tg21 mice at P21. Shown are all four trials per test. 2-way ANOVA, F(1,152) = 9.561, P = 0.0034. Multiple comparisons: **P = 0.0052. N = 19 animals per genotype. (L–N) Rotarod motor coordination tests across postnatal ages in Sox10-cre;EpoRfx/fx mice. (L) Average values from the four trials per animal at P14, P21 and P60. N = 8–15 animals per genotype and age. (M) Rotarod motor coordination tests in Sox10-cre;EpoRfx/fx mice at P14. Shown are all four trials per test. 2-way ANOVA, F(1,112) = 21.46, P < 0.0001. Multiple comparisons: *P = 0.0159, **P = 0.0055. N = 15 animals per genotype. (N) Rotarod motor coordination tests in Sox10-cre;EpoRfx/fx mice at P21. Shown are all four trials per test. N = 9 cre− and 8 cre+ animals.
