Table 1 Perfusion and brain cutting thickness parameters.
Age group | Pentobarbital volume (µl) | Needle size (G) | Pump rate (rpm) | Postfixation time (h) | Cut thickness (µm) | Series sample fraction (µm) |
|---|---|---|---|---|---|---|
P3 | 10 | 27 | Manual | 72 | 60 | 240 |
P7 | 20 | 26 | 0.4 | 24 | 50 | 300 |
P11 | 30 | 25 | 0.4 | 18 | 40 | 320 |
P14 | 40 | 25 | 0.5 | 12 | 40 | 400 |
P21 | 50 | 24 | 0.9 | 3 | 40 | 480 |
P25 | 100 | 24 | 1.2 | 3 | 40 | 480 |
