Table 1 Formulation and proximate composition of commercial (COM-A), plant-based (PB1 & PB2) and plant-based experimental feeds supplemented with insect hydrolysate, spirulina and yeast extract (ISY1 & ISY2) for rainbow trout feeding in Experiment A.

From: New alternative ingredients and genetic selection are the next game changers in rainbow trout nutrition: a metabolomics appraisal

Ingredients (g.100g–1 FW)

COMA

PB1

PB2

ISY1

ISY2

Fish meal

21.03

    

Fish oil

4.88

    

DHA-rich algae meal

 

6.84

6.84

6.84

6.84

Insect hydrolysate

   

5.00

5.00

Spirulina

   

5.00

5.00

Yeast extract

   

5.00

5.00

Processed animal proteins1

15.00

    

Vegetal oils2

14.65

18.10

17.75

16.75

16.50

Plant proteins3

42.7

70.60

70.53

57.14

56.95

Rapeseed lecithin

 

1.00

1.00

1.00

1.00

Monocalcium phosphate

 

1.20

1.25

1.05

1.10

Phytase

 

0.02

0.02

0.02

0.02

Lysine 78%

0.39

0.50

1.00

0.50

1.00

DL-methionine 98%

0.44

0.65

0.52

0.61

0.50

Threonine 98%

0.20

0.20

0.20

0.20

0.20

Vitamin premix4

0.25

0.30

0.30

0.30

0.30

Vitamin C monophosphate 35 %

0.04

0.04

0.04

0.04

0.04

Mineral premix5

0.25

0.30

0.30

0.30

0.30

Liquid choline

0.15

0.25

0.25

0.25

0.25

Antioxidant

0.10

0.10

0.10

0.10

0.10

Antifungal

0.15

0.15

0.15

0.15

0.15

Proximate composition6

 Dry matter (% FW)

95.8

96.9

94.6

96.9

96.5

 Proteins (% DM)

43.0

44.7

45.7

45.0

45.3

 Lipids (% DM)

21.0

21.6

21.4

21.2

21.4

 Ash (% DM)

7.3

5.4

5.4

6.3

5.9

 Energy (kJ.g–1 DM)

24.1

24.0

24.4

24.0

24.4

  1. 1Processed animal protein (feather meal protein, blood product, poultry meal, 5/3/7 g.kg–1).
  2. 2Vegetable oils: rapeseed oil and linseed oil.
  3. 3Plant proteins: COM: soy concentrate, soybean meal, rapeseed meal, peeled fava bean and wheat. PB1: wheat gluten, hydrolysed wheat gluten, pea protein, fava bean protein concentrate, soy concentrate, wheat. PB2: pea protein, corn gluten, soybean meal, guar meal, peeled fava bean. ISY1: a fraction of wheat gluten, pea protein, fava bean protein, soy concentrate and wheat as well as a fraction of rapeseed oil and linseed oil were substituted by the insect-spirulina-yeast fraction. ISY2: a fraction of pea protein, corn gluten, soybean meal, guar meal, peeled fava bean and wheat as well as a fraction of rapeseed oil and linseed oil were substituted by the insect-spirulina-yeast fraction.
  4. 4Vitamin premix composition: vitamin A (retinyl acetate/3a672a) 4 000 000 UI/kg; vitamin D (cholecalciferol/3a671) 700 000 UI/kg; vitamin E (alpha-tocopheryl acetate/3a700) 80 000 UI/kg; vitamin K3 (menadione/3a711) 4 g/kg; vitamin B1 (thiamine mononitrate/3a821) 4 g/kg; vitamin B2 (riboflavin) 6 g/kg; vitamin B6 (pyridoxine hydrochloride/3a831) 6 g/kg; vitamin B12 (cyanocobalamin) 20 mg/kg; vitamin B5 (D-calcium pantothenate/3a841) 12 g/kg; nicotinic acid (vitamin PP—B3—niacin 3a314/niacinamide 3a315) 12 g/kg; folic acid (vitamin B9/3a316) 3.6 g/kg; biotin (vitamin B8/3a880) 0.4 g/kg.
  5. 5Mineral premix composition: iodine (calcium iodide anhydrous/3b202) 0.4 g/kg; manganese (manganese oxide II/3b502) 20 g/kg; zinc (zinc oxide/3b603); 40 g/kg; iron (iron II sulfate monohydrate/3b103) 32 g/kg; copper (copper II sulfate pentahydrate/3b405) 1.2 g/kg.
  6. 6FW fresh weight, DM dry matter.
Back to article page