Table 5 Annotated spectra regions of metabolites from Experiment-B for which diet, line selection or interaction between diet and line selection had a significant effect (two-way ANOVA P <0.05) on its relative intensity.

From: New alternative ingredients and genetic selection are the next game changers in rainbow trout nutrition: a metabolomics appraisal

Spectrum region_metabolite

Diet P-value

Line selection P-value

Diet x Line P-value

B1.0221_isoleucine*

4.20E-02

5.64E-01

6.65E-01

B1.1529_1,2-propanediol*

3.35E-05

1.10E-01

7.05-02

B1.1874_ethanol

8.69E-04

4.59E-02

3.37E-01

B1.4757_alanine*

3.11E-02

3.19E-01

2.98E-01

B2.6485_methionine

9.61E-07

9.67E-01

5.73E-01

B2.9323_dimethylglycine+Unk

3.13E-04

9.98E-01

3.62E-01

B3.0166_lysine*

7.75E-03

4.01E-01

6.74E-01

B3.3540_methanol*

1.52E-02

2.38E-02

9.24E-01

B3.4965_glucose

6.80E-06

3.98E-02

9.51E-01

B3.5627_glycine

1.49E-04

1.09E-01

7.14E-01

B3.9046_glycine-betaine*

7.67E-09

5.79E-01

4.52E-02

B3.9649_serine

3.38E-02

6.00E-01

2.02E-01

B4.0669_Inositol*

1.29E-07

6.77E-03

8.05E-01

B7.4330_phenylalanine*

3.62E-03

8.76E-01

1.39E-01

zB0.6680_cholesterol

1.76E-02

6.62E-01

6.17E-02

zB0.9210_VLDL_LDL

2.41E-11

1.67E-01

4.41E-01

zB1.2495_VLDL_LDL

5.71E-03

1.01E-01

2.22E-01

zB1.7440*

5.16E-09

2.71E-01

8.94E-01

zB1.8110

2.55E-04

1.41E-01

7.80E-01

zB2.0137

4.23E-03

2.53E-01

4.67E-01

zB2.7400_unsaturated_FA*

6.83E-05

9.28E-01

4.121E-01

zB2.8109_unsaturated_FA

1.17E-02

1.30E-01

1.03E-01

zB3.2325

2.61E-03

9.40E-01

8.58E-01

zB4.3205_Ɛ_Glyceryl

2.13E-05

1.65E-01

8.71E-01

  1. Twenty-four out of the 37 selected spectra regions representing all the annotated compounds and lipid signatures in plasma of rainbow trout fed a plant-based diet supplemented with insect and yeast (IY3) ingredients compared to a commercial-like diet (COM-B) in a line selected (S) for plant-based diets and a non-selected line (NS). P-values < 0.05 are in bold.
  2. *Log2-transformed variables prior to ANOVA analysis.