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Figure 1

From: The common H232 STING allele shows impaired activities in DNA sensing, susceptibility to viral infection, and in monocyte cell function, while the HAQ variant possesses wild-type properties

Figure 1

IFN and NF-kB pathway activities after different stimuli. THP-1 cell lines stably express a Secreted Luciferase (SLuc) and Secreted Embryonic Alkaline Phosphatase (SEAP) reporter genes respectively under the control of IFN and NF-kB responsive promoters. All cell lines were stimulated with a DNA stimulus or with a STING-unrelated stimulus (3p-hp-RNA). Luciferase (A) and SEAP (B) activities were assessed 24 h post stimulus from supernatants. Lipofectamine panels show the basal expression. Activation of IFNb pathway upon PMA stimulus was depicted in panel (C). Triggering of IFNb upon 2′3′cGAMP is shown in panel (D). The statistical analysis was performed by student’s t-test by multiple comparisons. Dashed lines indicate p < 0.005; solid line p < 0.05.

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