Figure 2

IFNβ transcriptional activation in differentiated macrophages. THP-1 cell lines were treated with phorbol myristate acetate (PMA) alone or with PMA in combination with a DNA stimulus. After 18 h the transcriptional activation of endogenous IFNβ was evaluated into differentiated macrophages. Functional rescue was performed using supplementing recombinant IFNβ in the supernatant of STING KO cells (white bar). The statistical analysis was performed by student’s t test using NT of each allele as reference.