Figure 2
Effect of NOS3 silencing on tracer uptake in HDMVECs and HRECs. (a) Representative images of dextran-Texas Red uptake in HDMVECs and HRECs treated with siNT and siNOS3. Cells were left unstimulated or treated with VEGF-A (25 ng/mL) for 48 h prior to the addition of the tracer. Quantification of dextran uptake is shown in (b), in n = 4 independent experiments. (c) Representative images of BSA-FITC uptake in HDMVECs and HRECs treated with siNT and siNOS3. (d) Quantification of BSA-FITC uptake in images shown in (d), n = 3 independent experiments. All results are normalized to the unstimulated siNT control. Scale bars: 20 µm. Data are represented as mean ± SEM.
