Figure 4
VEGFR2 expression is regulated by eNOS in HUVECs, but not in HDMVECs and HUVECs. (a) Relative KDR mRNA levels in control and siNOS3-treated HDMVECs, HRECs and HUVECs at 72 h after transfection, in n = 3–4 independent experiments. (b) Representative cropped images of western blots of VEGFR2 and β-actin (loading control) expression in HDMVECs, HRECs and HUVECs transfected with siNT or siNOS3. Cells were cultured in the presence or absence of VEGF-A (25 ng/mL for 48 h). Original blots are presented in Supplementary Figs. S2, S7 and S8. (c) VEGFR2 protein levels in control and in siNOS3-treated HDMVECs, HRECs and HUVECs at 72 h after siRNA transfection, in n = 3–4 independent experiments. (d) KDR mRNA levels in HDMVECs, HRECs and HUVECs after repeated treatment with NO donor SNAP (50 µM, 100 µM or 200 µM) over a 48 h incubation period (cells were stimulated 3 times for 4 h per day with freshly prepared SNAP), in n = 3–4 independent experiments. Data are represented as mean ± SEM. *p < 0.05; **p < 0.01 (one-sample t-test or Student’s t-test).
