Table 6 Primers sequences for the real-time quantitative reverse transcription-polymerase (RT-qPCR).

From: RETRACTED ARTICLE: Sciadopitysin mitigates spermatological and testicular damage instigated by paraquat administration in male albino rats

Gene

Primers 5'ā€”>ā€‰3'

Accession number

3Ī²-HSD

Forward: GCATCCTGAAAAATGGTGGC

NM_001007719

Reverse: GCCACATTGCCTACATACAC

17Ī²-HSD

Forward: CAGCTTCCAAGGCTTTTGTG

NM_054007

Reverse: CAGGTTTCAGCTCCAATCGT

StAR

Forward: AAAAGGCCTTGGGCATACTC

NM_031558

Reverse: CATAGAGTCTGTCCATGGGC

Bax

Forward: GGCCTTTTTGCTACAGGGTT

NM_017059.2

Reverse: AGCTCCATGTTGTTGTCCAG

Bcl-2

Forward: ACAACATCGCTCTGTGGAT

NM_016993.1

Reverse: TCAGAGACAGCCAGGAGAA

Caspase-3

Forward: ATCCATGGAAGCAAGTCGAT

NM_012922.2

Reverse: CCTTTTGCTGTGATCTTCCT

Ī²-actin

Forward: TACAGCTTCACCACCACAGC

NM_031144

Reverse: GGAACCGCTCATTGCCGATA

  1. Ī²-HSD: 3Ī²-hydroxysteroid dehydrogenase; 17Ī²-HSD: 17Ī²-hydroxysteroid dehydrogenase; StAR: Steroidogenic acute regulatory protein; Bcl-2: B-cell lymphoma 2; Bax: Bcl-2-associated X protein; Caspase-3: Cysteineā€“aspartic acid protease.
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