Figue 1
From: Orai1α and Orai1β support calcium entry and mammosphere formation in breast cancer stem cells
Expression of Orai and TRPC members in breast stem cells and breast cancer stem cells derived from the MCF10A, MCF7, SKBR3 and MDA-MB-231 cell lines. (A) RT-qPCR expression analysis of Orai1, Orai2, Orai3, TRPC1, TRPC3 and TRPC6 mRNA transcripts in BSC derived from the MCF10A cell line and BCSC derived from the MCF7, SKBR3 and MDA-MB-231 cell lines. Values were normalized to GAPDH expression and represented as mean expression relative to MCF10A-derived BCSC ± S.E.M.; n = 6. (B) Whole cell lysates from non-stem cells and stem cells derived from MCF10A, MCF7, SKBR3 and MDA-MB-231 cell lines were subjected to 10% SDS-PAGE and Western blotting with specific anti-Orai1 antibody, as indicated. Blots were reprobed with anti-β-actin antibody for protein loading control. Bar graph represents Orai1 expression presented as mean ± SEM. Data were statistically analyzed using Kruskal–Wallis test with multiple comparisons (Dunn´s test). **p < 0.01 and ****p < 0.0001.
