Figure 1
From: Temperature modulates the osmosensitivity of tilapia prolactin cells
Effects of incubation osmolality and temperature on Prl188 (A and B) and Prl177 (C and D) release from Prl cells of SW-acclimated (A and C) and FW-acclimated (B and D) Mozambique tilapia following 1 h and 6 h of incubation. Data are expressed as µg/105 cells ± SEM (n = 6–8). The effects of osmolality and temperature at each time point were analyzed by two-way ANOVA (*P < 0.05, **P < 0.01, ***P < 0.001). When there was a significant effect of temperature (Temp), media osmolality (Osm) or interaction (Int), group comparisons were conducted using protected Fisher’s LSD test. Groups not sharing uppercase letters indicate significant (P < 0.05) mean differences in response to incubation temperatures and groups not sharing lowercase letters reflect significant (P < 0.05) mean differences in response to media osmolality. Grey dashed lines represent baseline control Prl release (330 mOsm/kg:26 °C) after 1 h (0.04 and 0.02 µg/105 cells for Prl188 and Prl177 released from SW fish, respectively and 0.20 and 0.10 µg/105 cells for Prl188 and Prl177 released from FW fish, respectively) and grey dotted lines represent baseline Prl release after 6 h (0.08 and 0.06 µg/105 cells for Prl188 and Prl177 released from SW fish, respectively and 1.04 and 0.41 µg/105 cells for Prl188 and Prl177 released from FW fish, respectively).
