Figure 5
From: Temperature modulates the osmosensitivity of tilapia prolactin cells
Effects of incubation osmolality and temperature on the mRNA expression of pou1f1 (A), pou2f1b (B), creb3l1 (C), cebpb (D), stat3 (E), stat1a (F) and nfatc1 (G) in SW-acclimated tilapia Prl cells after 6 h of incubation. Data are expressed as mean fold change from the isosmotic and isothermal (330 mOsm/kg:26 °C) group ± SEM (n = 6–8). The effects of osmolality and temperature were analyzed by two-way ANOVA (*P < 0.05, **P < 0.01, ***P < 0.001). When there was a significant effect of temperature (Temp), media osmolality (Osm) or interaction (Int), group comparisons were conducted using protected Fisher’s LSD test. Groups not sharing uppercase letters indicate significant (P < 0.05) mean differences in response to incubation temperatures and groups not sharing lowercase letters reflect significant (P < 0.05) mean differences in response to media osmolality.
