Table 1 Gene specific primers used for qPCR.
From: Temperature modulates the osmosensitivity of tilapia prolactin cells
Gene | Primer sequence (5′–3′) | R2 | Efficiency % | Accession number | References |
|---|---|---|---|---|---|
18s | F: GCTACCACATCCAAGGAAGGC R: TTCGTCACTACCTCCCCGAGT | 0.993 | 70.717 | AF497908 | |
ef1a | F: AGCAAGTACTACGTGACCATCATTG R: AGTCAGCCTGGGAGGTACCA | 0.992 | 74.954 | AB075952 | |
β-actin | F: CTCTTCCAGCCTTCCTTCCT R: ACAGGTCCTTACGGATGTCG | 0.987 | 70.992 | FN673689 | |
pou1f1 | F: GGCAATGCTCTCAGCAACAC R: GCATCTCCTGTGCTGCCAT | 0.995 | 77.372 | XM_019352661.2 | |
stat3 | F: TATCTGCGTTACCCCGTGTC R: TTTGTGCCTGGGAATCCGTT | 0.985 | 84.833 | XM_013269621.3 | |
creb3l1 | F: CAGTTTAACAGCGGAGAAACTCTA R: GGTCACCTGAGAAAGGCACATT | 0.998 | 81.644 | XM_005460642.4 | |
stat1a | F: ACCATCAGAGGCTGCTGAAC R: CAGCCTGGACGGATGAACTT | 0.983 | 89.756 | XM_005452305.4 | |
pou2f1b | F: GGGGACAGATTGCTGGAGTA R: AGCTTCAGCCAAGTCATCGT | 0.997 | 82.643 | XM_025903751.1 | |
cebpb | F: CACATTCACACACCGGAGAC R: CCTGTGAAGCGTACCGTTTT | 0.992 | 91.534 | XM_003438913.5 | |
nfatc1 | F: GCCGCTGTAGCTTTAAGTGG R: ACACTGAGGCGAGCTCAAAT | 0.997 | 96.122 | XM_003447265.5 | |
prl177 | F: TGGTTTGGCTCTTTTAACACAGTG R: AGACAATGAGGAGTCACAGAGATTTTAC | 0.998 | 92.719 | M27011 | |
prl188 | F: GGCCACTCCCCATGTTTAAA R: GGCATAATCCCAGGAGGAGAC | 0.998 | 95.252 | X93280 | |
prlr1 | F: TGGGTCAGCTACAACATCACTGT R: GGATGGGGCTTGACAATGTAGA | 0.983 | 71.697 | EU999785 | |
prlr2 | F: GCCCTTGGGAATACATCTTCAG R: GTGCATAGGGCTTCACAATGTC | 0.993 | 87.509 | EU999783 | |
trpv4 | F: AGTGGAGCCCATCAATGAG R: TGTGGTATGTGGGTATGGAG | 0.983 | 90.702 | AB648937 |
