Table 3 Variance component analysis results for principal components of ‘L’ phrases in Oriental tit and Varied tit responses to plastic snake. This table represents the results of Variance Component Analysis (conducted using the ‘VCA’ function in the R package ‘VCA’) for varimax-rotated principal components derived from 13 characteristics of ‘L’ phrases (PC1, PC2, PC3, PC4; see Supplementary Table S3). The analysis assesses the proportion of variance in each principal component explained by the species during plastic snake presentation experiments for the two species, recorded with a Primo EM172 microphone (see Table 4 in Methods). The smaller proportion of total variance explained by the species (indicated in bold underlined font) indicates the larger degree of overlap in acoustic properties between calls of the two species given in response to the plastic snake. The error can be interpreted as a within-trial variation of the principal component. This table corresponds to Figs. 3A & 3B.

From: Unlearned adaptive responses to heterospecific referential alarm calls in two bird species from separate evolutionary lineages

Principal Component

Levels of variance

df

Variance

% to total variance

SD

PC1: Frequency pitch

(24%)

(pitch)

Total

3.54

12.84

100

3.58

Species

1

6.30

49.08

2.51

Species/nest

19

4.05

31.60

2.01

Error

589

2.47

19.30

1.57

PC2

(phrase duration, syllable number, call rate variability)

Total

127.49

5.96

100

2.44

Species

1

0.30

5.15

0.55

Species/nest

19

1.00

16.79

1.00

Error

589

4.65

78.05

2.15

PC3

(syllable duration & its variability, call rate)

Total

254.54

4.95

100

2.22

Species

1

0

0

0

Species/nest

19

1.02

20.63

1.01

Error

589

3.93

79.36

1.98

PC4

(range & pitch variability)

Total

9.25

4.00

100

2.00

Species

1

1.19

29.85

1.09

Species/nest

19

0.88

22.01

0.93

Error

589

1.92

48.13

1.38