Figure 4

Disrupted foliation pattern in the developing cerebellum of Adk-tg mice. (A) Nissl-stained cerebellum of Adk-tg and WT mice at postnatal day 5 (P5). Black arrows pointed toward the bifurcation points in the vermis of the Adk-tg cerebellum. (B) Nissl-stained sections from Adk-tg and WT cerebellum at P15. High-magnification images of the Nissl-stained hemisphere sections from Adk-tg and WT control mice at P15 illustrate the difference in the thickness of the external granule layer (EGL) between both lines. (C) Immunofluorescence reactivity of Ki67 (green) in Adk-tg and WT control mice in cerebellar sections at P2, P6, and P8 illustrating changes in the thickness of EGL. (D) Quantitative analysis of Ki67 layer thickness in P2, P6, and P8 Adk-tg and WT mice cerebellum. (E) Immunofluorescence reactivity of calbindin (green) and ADK (red) in P9 and adult Adk-tg and WT control mice illustrating changes in Purkinje layer at P9 in ADK-tg. (F) Quantitative analysis of Purkinje layer thickness in Adk-tg and WT mice cerebellum at P9. Scale bar is 2 mm in A and B, 200 µm (yellow box) and100 µm in B, and 50 µm in C, and 100 µm in E. All values are presented as mean ± SEM (n = 3). In D, two-way ANOVA with Tukey's multiple comparison post-hoc test (*p = 0.02 and **p = 0.0013 for significance). In F, Unpaired 2-tailed t-test ****p = 0.0002 for significance).