Figure 2 | Scientific Reports

Figure 2

From: VarLOCK: sequencing-independent, rapid detection of SARS-CoV-2 variants of concern for point-of-care testing, qPCR pipelines and national wastewater surveillance

Figure 2

Detection specificity is low for single nucleotide substitution with standard conditions. A representative experiment showing collateral nuclease activities (y axis; arbitrary fluorescence unit increase/min) triggered by wt gRNA matching wt target (wild type detection), wt gRNA matching mutation target (wt cross-reaction), mutation gRNA matching wt target (mutation cross-reaction) and mutation gRNA matching mutation target (mutation detection). Mutations are indicated on the top of each panel. Average and SD are derived from technical duplicates.

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