Table 2 Primer sequences of TOP10 circRNA.
Primer | Accession number | Primer sequence (5′–3′) | Ta | Amplicon size |
|---|---|---|---|---|
GAPDH-F | NM_002046.7 | CAATGACCCCTTCATTGACC | 60 °C | 264 |
GAPDH-R | TTGATTTTGGAGGGATCTCG | |||
hsa_circ_0091581-F | NM_001164617 | GACCACCACTAGGCCTTTGAA | 695 | |
hsa_circ_0091581-R | CTTGTGGAGTCAGGCTTGGG | |||
hsa_circ_0079875-F | NM_018685 | AGAAACCAAACAGGAGGAACAGG | 299 | |
hsa_circ_0079875-R | TTCTGTGGGGTGTGCTACGA | |||
hsa_circ_0052585-F | NM_001165931 | TGCCTGTGAAGCTCATTGGGA | 219 | |
hsa_circ_0052585-R | CCAGGTGTTTGAACATCAGGCA | |||
hsa_circ_0059730-F | NM_012112 | ACGGAGAGAACTGGGTGTTCC | 720 | |
hsa_circ_0059730-R | TGCAAAGGCTGGTGACTTGG | |||
hsa_circ_0004788-F | NM_133631 | GAAGTAGCCAGCTCCCGTCT | 327 | |
hsa_circ_0004788-R | CGGCCTTCAGCTTTGCAGTT | |||
hsa_circ_0060361-F | NM_030919 | GCTCCTACAGAAAGCAGCCAA | 907 | |
hsa_circ_0060361-R | GACAAAAACCGGCGTGCAG | |||
hsa_circ_0007901-F | NM_018407 | ACACTACGACATGTGCATTGCC | 392 | |
hsa_circ_0007901-R | TGTACGCTCCGTAAGTAGCCA | |||
hsa_circ_0002514-F | NM_002073 | GGATAACCAGACAGTGAAGGGC | 1172 | |
hsa_circ_0002514-R | CTGGTTGCTGTGGCGATGTT | |||
hsa_circ_0001135-F | NM_012112 | TCGAGAAATTGCAACAAAGGTGA | 1266 | |
hsa_circ_0001135-R | CCGAGGGGGCATCATAGGAA |
