Figure 2 | Scientific Reports

Figure 2

From: Cryopreservation of rat embryos at all developmental stages by small-volume vitrification procedure and rapid warming in cryotubes

Figure 2

Survival and in vitro development of cryopreserved one-cell embryos in a sample volume of 15, 30, 50, or 100 μL, which were cooled using direct immersion in liquid nitrogen (LN2) at a rate of 7950, 7160, 6800 and 5830 °C/min, respectively, and warmed with sucrose solution at 50 °C. The warming rates were 68,850, 49,400, 44,500 and 35,480 °C/min, respectively. Each bar indicates the percentage of embryos surviving (open bars), developing to the two-cell stage (shaded bars), and developing to the blastocyst (closed bars) after cryopreservation (N = 8–10 experimental runs); numbers in parentheses indicate the number of one-cell embryos examined. Data are shown as means ± SEM. Different lowercase letters indicate a significant difference at P < 0.05.

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