Figure 4 | Scientific Reports

Figure 4

From: Cryopreservation of rat embryos at all developmental stages by small-volume vitrification procedure and rapid warming in cryotubes

Figure 4

In vivo development of cryopreserved one-cell rat embryos. The one-cell embryos were vitrified with 5% PG for 10 min and PEPeS for 40 s. The embryos were warmed using 1 mL of sucrose solution at 50 °C. The cryopreserved or fresh embryos were transferred into the oviducts of pseudopregnant female rats. (A) Offspring survival. Numbers in parentheses indicate the number of one-cell embryos examined. Data are shown as means ± SEM. Different lowercase letters indicate a significant difference at P < 0.05. (B) Live pups derived from vitrified one-cell embryos.

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