Figure 5
(A) Thermal cycle oscilloscope trace of a 15 μL aqueous sample (PEPeS) in vitrification solution24 in a cryotube equipped at the bottom with a 50 μm Cu-constantan thermocouple. The cryotube sample was rapidly moved from room-temperature air (~ 23 °C) to LN2 and held for 5 s, and then held in air at 23 °C for 1 min before warming by the addition of 1 mL of sucrose solution at 50 °C. (B) Details of events during warming when the sample was warmed by adding 1 mL of sucrose solution at 50 °C. A straight line visually fit to the data revealed a warming rate of 84,000 °C/min. The temperature sampling rate was 500 readings/sec.
