Table 2 Cooling and warming conditions.

From: Cryopreservation of rat embryos at all developmental stages by small-volume vitrification procedure and rapid warming in cryotubes

Volume of 5% PGa + PEPeS (μL)

Cooled by

Cooling rate ± SEM (°C/min)

Temperature of sucrose (0.3 M) warming solution (1 mL) (°C)

Warming rate ± SEM (°C/min)

15

LN2

7950 ± 210 (N = 8b)

23

29,930 ± 2180 (N = 8)

37

38,780 ± 2010 (N = 8)

50

68,850 ± 5670 (N = 8)

30

7150 ± 105 (N = 8)

50

49,400 ± 2860 (N = 8)

50

6800 ± 320 (N = 8)

50

44,500 ± 3520 (N = 8)

100

5830 ± 250 (N = 8)

50

35,480 ± 1320 (N = 8)

  1. aPG, propylene glycol; PEPeS, 10% v/v PG, 30% v/v ethylene glycol, 0.3 M sucrose, and 20% v/v Percoll in phosphate-buffered saline); LN2, liquid nitrogen.
  2. bRefers to the number (N) of experimental run.
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