Table 2 Mock community experimental design.

From: Mock community experiments can inform on the reliability of eDNA metabarcoding data: a case study on marine phytoplankton

Group

Mock

Dinoflagellates

Diatoms

Alexandrium minutum cell number

Alexandrium pacificum cell number

Scrippsiella spp. cell number

Chaetoceros socialis cell number

Pseudo-nitzschia spp. cell number

Skeletonema marinoi cell number

Thalassionema frauenfeldii cell number

1

1

3.3 × 105

0

0

3.3 × 105

3.3 × 105

0

0

2

3.3 × 105

0

3.3 × 105

3.3 × 105

0

0

0

3

2.0 × 105

2.0 × 105

2.0 × 105

2.0 × 105

2.0 × 105

0

0

4

2.0 × 105

0

2.0 × 105

2.0 × 105

0

2.0 × 105

2.0 × 105

5

1.6 × 105

1.6 × 105

1.6 × 105

1.6 × 105

1.6 × 105

1.6 × 105

0

6

1.6 × 105

1.6 × 105

1.6 × 105

1.6 × 105

0

1.6 × 105

1.6 × 105

7

1.4 × 105

1.4 × 105

1.4 × 105

1.4 × 105

1.4 × 105

1.4 × 105

1.4 × 105

2

8

2.0 × 105

0

0

4.0 × 105

4.0 × 105

0

0

9

2.0 × 105

0

4.0 × 105

4.0 × 105

0

0

0

10

1.0 × 105

1.0 × 105

1.0 × 105

3.5 × 105

3.5 × 105

0

0

11

1.0 × 105

0

1.0 × 105

3.5 × 105

0

3.5 × 105

1.0 × 105

12

1.0 × 105

1.0 × 105

1.0 × 105

3.0 × 105

3.0 × 105

1.0 × 105

0

13

1.0 × 105

1.0 × 105

1.0 × 105

3.0 × 105

0

3.0 × 105

1.0 × 105

14

8.0 × 104

8.0 × 104

8.0 × 104

3.0 × 105

3.0 × 105

8.0 × 104

8.0 × 104

3

15

4.0 × 105

0

0

4.0 × 105

2.0 × 105

0

0

16

4.0 × 105

0

2.0 × 105

4.0 × 105

0

0

0

17

3.5 × 105

1.0 × 105

1.0 × 105

3.5 × 105

1.0 × 105

0

0

18

3.5 × 105

0

1.0 × 105

3.5 × 105

0

1.0 × 105

1.0 × 105

19

3.0 × 105

1.0 × 105

1.0 × 105

3.0 × 105

1.0 × 105

1.0 × 105

0

20

3.0 × 105

1.0 × 105

1.0 × 105

3.0 × 105

0

1.0 × 105

1.0 × 105

21

3.0 × 105

8.0 × 104

8.0 × 104

3.0 × 105

8.0 × 104

8.0 × 104

8.0 × 104

  1. Taxon abundance (number of cells) was reported for each taxon and mock community. Mock communities were grouped according to the scheme described in Materials and methods.