Figure 7
Influence of Tgfβ1 knockdown on lymphangiogenesis in LECs under hypoxia. Tgfb1 knockdown were performed by transfection with siRNA against Tgfb1 using two types of Tgfb1 siRNA (siRNA#1 and siRNA#2). (a) Expression level of Tgfβ1 in LECs after administration of CCN2 under hypoxia. The data are expressed as the mean ± standard error, n = 3/group. (b) Cellular proliferation of LECs after administration of CCN2 under hypoxia. The data are expressed as the mean ± standard error. The LECs were in passage 6, n = 5/group. (c) Levels of ERK 1/2, SMAD 2, SMAD 3, and SMAD 4 in LECs after administration of CCN2 under hypoxia. The median fluorescence intensity of these proteins was measured using the Luminex® system, n = 3/group. (d) Expression levels of Vegfc and Vegfr3 in LECs after administration of CCN2 under hypoxia. The data are expressed as the mean ± standard error, n = 3/group. *P < 0.05; **P < 0.01; ***P < 0.001. ns not significant, CCN2 cellular communication network factor 2, ERK extracellular signal-regulated kinase, LECs lymphatic endothelial cells, SMAD suppressor of mothers against decapentaplegic.
