Figure 4

Biochemical characterization of recombinant PCHepII. (A) Effect of temperature on PCHepII activity. The highest activity observed at 20°C on HP was set at 100%. (B) Effect of pH on PCHepII activity. The highest activity observed in the 50 mM sodium phosphate buffer (pH 7.0) on HP was set as 100%. (C) Effect of metal ions and chelators including Li⁺, K⁺, Na⁺, Ag⁺, Ca²⁺, Mg²⁺, Mn²⁺, Ni²⁺, Co²⁺, Hg²⁺, Cd²⁺, Zn²⁺, Pb²⁺, Fe²⁺, Ba²⁺, Cu²⁺, Fe³⁺, Cr³⁺, EDTA, imidazole, glycerol, SDS, β-mercaptoethanol and DTT on PCHepII activity. The enzyme activity on HP obtained without the inclusion of tested metal ions and chemicals was considered as 100%. (D) Effect of KCl concentration on PCHepII activity. The activity of PCHepII without LiCl was set as 100%. (E) Thermostability of PCHepII. The residual activity of PCHepII on HP without preincubation was set at 100%.