Figure 6
Polysaccharide degradation pattern analysis of recombinant PCHepII with HP as substrate. Purified PCHepII was used to treat HP (1 mg/mL) at 20°C in 50 mM sodium phosphate buffer (pH 7.0) for 0–24 h. Samples of 30 μg were taken at intervals for gel filtration HPLC analysis. The degree of depolymerization of HP unsaturated oligosaccharides released from the polysaccharide substrates by the digestion of HP are indicated by arrows: Hexa, HP unsaturated hexasaccharide; Tetra, HP unsaturated tetrasaccharide; Di-3S, the trisulfated HP unsaturated disaccharides; Di-2S, the disulfated HP unsaturated disaccharides.
