Figure 9 | Scientific Reports

Figure 9

From: Identification and characterization of a novel heparinase PCHepII from marine bacterium Puteibacter caeruleilacunae

Figure 9

Simulated structure (A) and mutagenesis analysis (B) of PCHepII. (A) The structure of PCHepII was simulated via the Swiss Model online software. (B) The mutants PCHepII-H199A, PCHepII-Y254A, and PCHepII-H403A were applied to treat HP for overnight and then analyzed by the gel filtration HPLC. Hexa, HP unsaturated hexasaccharide; Tetra, HP unsaturated tetrasaccharide; Di-3S, the trisulfated HP unsaturated disaccharides; Di-2S, the disulfated HP unsaturated disaccharides.

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