Figure 1 | Scientific Reports

Figure 1

From: Generation of “OP7 chimera” defective interfering influenza A particle preparations free of infectious virus that show antiviral efficacy in mice

Figure 1

Plasmid-based reconstitution of OP7 chimera DIPs free of infectious STVs. Rescue of Seg 1 conventional DIPs (cDIPs). The reverse genetics system comprises (A) eight plasmids that encode for a deleted Seg 1 vRNA and Seg 2–8 wild type (WT) vRNAs. (B) Co-transfection of a co-culture of PB2-expressing HEK-293T-PB2(adh) (high transfection efficiency) and MDCK-PB2(adh) (high virus titers) cells results in reconstitution of (C) clonal Seg 1 cDIP free of infectious standard viruses (STV). Rescue of OP7 chimera DIPs: addition of a (D) ninth plasmid that encodes for the mutated Seg 7-OP7 vRNA results in the reconstitution of a mixture of DIPs including (E) OP7 chimera DIPs and (C) Seg 1 cDIPs. This mixture of viruses can be propagated in MDCK-PB2(sus) cells (Fig. 2). Image was created with BioRender.com.

Back to article page