Figure 3

Effects of hypobaric hypoxia (HH) or normoxia dietary restriction (DR) for 7 days on the ghrelin levels and iron metabolism. (A) Changes in body weight of mice after 35% DR under normobaric normoxia or HH exposure. (B,C) The contents of ghrelin and acylated ghrelin in the plasma of mice after DR or HH treatment for 7 days. (D,F) Western blotting analysis of TfR, Fpn and Ft-L expression in the liver. (E,G) Western blotting analysis of TfR, Fpn and Ft-L expression in the spleen. Values are displayed as the mean ± SEM (n = 6 mice), * P < 0.05, ** P < 0.01, *** P < 0.001 versus the indicated group.