Figure 7 | Scientific Reports

Figure 7

From: Hypobaric hypoxia induces iron mobilization from liver and spleen and increases serum iron via activation of ghrelin/GHSR1a/MAPK signalling pathway in mice

Figure 7

Effects of ghrelin on expression of iron-regulating proteins and Erk phosphorylation in mouse hepatocytes under hypoxia condition. (A,F) Primary hepatocytes of mice were pretreated with a GHSR1a receptor antagonist (DLG, 100 nM) or MAPK signalling pathway inhibitor (U0126) for 1 h, followed by 1% O2 treatment with or without the presence of ghrelin (10–7 M) for 12 h. The relative expression levels of TfR, Ft-L, Fpn, pErk/ErK were detected by WB. (B–E,G–J) Semiquantitative analysis of TfR, Ft-L, Fpn, pErk/ErK in panels (A) and (F). Values are displayed as the mean ± SEM (n = 3 independent experiments), * P < 0.05, ** P < 0.01, *** P < 0.001 versus NN or the indicated group.

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