Figure 8 | Scientific Reports

Figure 8

From: Hypobaric hypoxia induces iron mobilization from liver and spleen and increases serum iron via activation of ghrelin/GHSR1a/MAPK signalling pathway in mice

Figure 8

Effects of ghrelin on expression of iron-regulating proteins and Erk phosphorylation in mouse peritoneal macrophages under hypoxia. (A,F) Peritoneal macrophages of mice were pretreated with GHSR1a receptor antagonist (DLG, 100 nM) or MAPK signalling pathway inhibitor (U0126) for 1 h, followed by 1% O2 treatment with or without the presence of ghrelin (10–7 M) for 12 h. The expressions of TfR, Ft-L, Fpn, pErk/ErK were detected by WB; (B–E,G–J) Semiquantitative analysis of TfR, Ft-L, Fpn, pErk/ErK in panels (A) and (F). Values are displayed as the mean ± SEM (n = 3 independent experiments), * P < 0.05, ** P < 0.01, *** P < 0.001 versus NN or indicated group.

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