Figure 2
From: FilGAP regulates tumor growth in Glioma through the regulation of mTORC1 and mTORC2
Analysis of FilGAP and mTORC1/2 association region. (A) Schematic diagram of FilGAP deletion mutants used to examine the interaction with Raptor. (B) Cell lysates prepared from Myc-Raptor transfected HEK293T cells were incubated with GST-FilGAP bound beads. Coprecipitated Myc-Raptor and mTOR were detected by immunoblotting. (C) Coprecipitated Raptor in (B) was quantified. (D) Schematic diagram of FilGAP deletion mutants used to examine the interaction with Rictor. The red dotted line is the estimated Rictor binding region. (E) Cell lysates prepared from Flag-Rcitor transfected HEK293T cells were incubated with GST-FilGAP bound beads. Coprecipitated Flag-Rictor was detected by immunoblotting. (G) Schematic diagram of Raptor deletion mutants. (H) Raptor NT and CT was coprecipitated with GST-FilGAP 373-748aa. (I) Flag-Rictor or Flag-Sin1 was transfected to HEK293T cells and cell lysates were subjected to pull down assay using GST-FilGAP 455-600aa. (J) Schematic diagram of Sin1 deletion mutants. (K) Purified MBP-Sin1 Full and NT was pulled down by GST-FilGAP 455-600aa.
