Figure 6
From: FilGAP regulates tumor growth in Glioma through the regulation of mTORC1 and mTORC2
FilGAP is involved in PI3K-independent mTORC2 activation in glioblastoma cells. (A) Immunoblots for FilGAP expression and mTORC1/2 activities in astrocytoma KINGS-1 and glioblastoma U-87MG cells. (B) P-S6K/Total S6K, P-AKT/Total AKT ratio and amount of FilGAP expression in (A) were calculated (n = 3). (C) U-87MG cells were transfected with siRNAs against FilGAP. (D) P-S6K/Total S6K and P-AKT/Total AKT ratio in (C) were calculated (n = 3). (E) Control or FilGAP-depleted U-87MG cells were treated with 10 μM LY294002 for 1 h. (F) P-AKT/Total AKT ratio in (E) were calculated (n = 5). *P < 0.05, **P < 0.01 (Student’s t-test).
