Figure 3
From: MarrowCellDLD: a microfluidic method for label-free retrieval of fragile bone marrow-derived cells
Fluid Dynamic MarrowCellDLD Sorting of Induced-OP9 Cells. (A) Trajectories of cells entering (inlet) and exiting (outlets) the microfluidic system. The test involved injecting induced-OP9 cells after 6 days of differentiation at a concentration of 500,000 cells/mL. (B) Phase contrast microscopy of outlet fractions after MarrowCellDLD sorting an induced-OP9 mixture. (C) Cell diameter distributions of outlet fractions (« small cells » fraction, violet; « large cells » fraction, yellow) after sorting induced-OP9s by MarrowCellDLD with a 19 µm separation cutoff. Phase contrast microscopy of cells collected at the outlets and replated after sorting as in (B) enabled cell size quantification in QuPath0.3.2. Data are displayed as relative frequencies over 150 cells per fraction from n = 4 sorting experiments. (D) ImageStream flow cytometry imaging (brightfield and LipidTOX-stained) of cells from the two fractions separated by MarrowCellDLD. MarrowCellDLD sortings were performed with a 19 µm separation cutoff and applied pressure of 20 mbar.
