Figure 4

Cell viability and functionality of OP9 cells after MarrowCellDLD sorting. (A) Cell Viability measurements: (i) pre-sorting, (ii) post-sample preparation, (iii) unsorted leftover, and (iv) cells collected at outlets post-sorting (small and large cell fractions). Statistical significance was evaluated by the Student’s t-test for independent samples (n = 15 MarrowCellDLD sorting experiments on induced-OP9 samples). (B–F) Time-sequential images at different days post-plating following MarrowCellDLD sorting of undifferentiated OP9 cells. Phase contrast and fluorescence (Yellow, LipidTOX staining) images for control cells (unsorted), unsorted leftover (unsorted sample residual at the inlet reservoir after 3 h sorting), and sorted (collected at the «small cells » fraction outlet) after induced adipocytic differentiation for 6 days. Scale bar: 100 µm. MarrowCellDLD sortings were performed with a 19 µm separation cutoff and applied pressure of 20 mbar.