Figure 5

Comparison between MarrowCellDLD and FACS sorters BD FACSAria™ III and MoFlo Astrios EQ. MarrowCellDLD size-based separation is compared to FACS sorting based on LipidTOX staining for lipidic content. (A, C) Post MarrowCellDLD or FACS sorting, cells from the unsorted sample and the sorted fractions were imaged by ImageStream flow cytometry. Distributions of sizes of single viable cells of each fraction after sorting by MarrowCellDLD, FACS Aria, and MoFlow Astrios FACS instruments. Cell diameters are extracted by ImageStream analysis for each method (200 cells per group or total cells analyzed in the fraction plotted from a single experiment, selected from n = 2 biological replicates using BD FACSAria™ III cell sorter and n = 3 biological replicates using MoFlo Astrios EQ cell sorter). Data are displayed with standard deviation as error bar, and box plot representing the 25% to 75% data points of the total sample. (B, D) Representative ImageStream images of brightfield and LipidTOX-stained cells from the FACS sorted fractions obtained by FACS Aria and MoFlo Astrios, respectively. MarrowCellDLD sortings were performed with a 19 µm separation cutoff and applied pressure of 20 mbar.