Figure 4
From: A human bispecific antibody neutralizes botulinum neurotoxin serotype A
ELISA results of LUZ-A1-A3 binding to the fragments of BoNT/A. The assay plates were coated with AHc (A), AHc-C (B) and AL-HN (C) expressed by E. coli. The serial dilution concentrations of the recombinant proteins were 400 ng/well, 200 ng/well, 100 ng/well, or 50 ng/well. The initial concentrations of LUZ-A1-A3 were 30 µg/mL, which were three-fold serially diluted in blocking buffer. Every concentration had three replicates. This result was one of three repeated assays. AHc, Hc domain of botulinum neurotoxin serotype A.
