Table 4 Neutralization efficiency of antibodies in vitro.

From: A human bispecific antibody neutralizes botulinum neurotoxin serotype A

Group

Number of survivorsa/total mice per group

IU/mg

125 μg

25 μg

5 μg

1 μg

0.2 μg

0.04 μg

HMAb A1

4/4

2/4

0/4

0/4

0/4

ND

0.4

HMAb A3

ND

4/4

2/4

0/4

0/4

0/4

2

A1 + A3b

ND

4/4

4/4

4/4

0/4

0/4

14

LUZ-A1-A3

ND

4/4

4/4

4/4

4/4

2/4

250

LUZ-8F2-6B1

0/10

ND

ND

ND

ND

ND

0

  1. ND: not determined.
  2. aThe serially diluted antibodies were mixed with 100 × LD50 of BoNT/A and incubated for 1 h at room temperature. Mice (n = 4 per group) were then intraperitoneally injected with the antibody toxin cocktail. The data show the number of surviving mice (survivors).
  3. bThe group A1 + A3 was mixed evenly in equal proportion by HMAb A1 and HMAb A3. For example, 125 μg means 62.5 μg HMAb A1 and 62.5 μg HMAb A3. An irrelevant antibody control, which was specific to dengue virus, served as negative control. The antibodies’ antitoxin efficacies were calculated as IU/mg, 1 IU/mg means 1 mg antibody neutralize 10,000 × LD50 of BoNT/A.
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