Figure 4
From: Rhythmic profile of memory T and B-cells along childhood and adolescence
Panoramic signatures of memory T and B-cells along childhood and adolescence. (A) The overall signature of memory T-cell subsets (CD4+ and CD8+) and B-cells was assembled for healthy children and adolescents (ALL (Green color bar), n = 812), categorized according to distinct age groups (9Mths–1Yr, n = 135; 2Yrs, n = 147; 3–4Yrs, n = 129; 5–7Yrs, n = 133; 8–10Yrs, n = 140 and 11–18Yrs, n = 128). Immunophenotypic analysis of NAIVE (N), Early EFFECTOR (eEF), CENTRAL (CM) and EFFECTOR MEMORY (EM) T-cell subsets as well as NAIVE (N), Early EFFECTOR (eEF), NON-CLASSICAL MEMORY (nCM) and CLASSICAL MEMORY (CM) B-cells was carried out by flow cytometry as described in Material and Methods. Signatures were assembled by first converting the original data, expressed as continuous variables (% of gated cells), into categorical results reported as proportion (%) of subjects with results above the cut-off edges (global median values of each cell subset). The final data are shown in radar chart, with each axis representing one memory cell subset. Cell phenotypes with proportion higher than the 50th percentile (gray zone) were underscored by *. (B) Heatmaps constructs were built to further illustrate the rhythmic distribution patterns of memory T-cell subsets and B-cells. Data are expressed as equalized distribution of subjects with results above the global median cut-off based on the color key provided in the figure. A white line set on the 50th percentile of data distribution was used to define the overall rhythm of signatures of memory T-cell subsets and B-cells.
