Figure 2

Scleral fibroblasts (SFs) exhibit myofibroblast transition during sclera maturation. SFs from young (four-week-old) and mature (six-month-old) pigmented guinea pigs were used in the study. (A) SFs were probed for α-SMA (green), Vimentin (red), and DAPI (blue). Scale bar: 20 μm. (B) Western blot analysis was performed to probe for Ki67 (359 kDa), collagen 1 (139 kDa), MMP2 (75 kDa), and α-SMA (42 kDa). GAPDH was used as an internal control. (C) Quantification of the protein expression levels in (B). The fold change relative to the control group level is displayed. **P < 0.01, *P < 0.05, n = 3. (D) Cell proliferation was assessed by CCK-8 assay after culture for 24 h, 48 h, and 72 h. ***P < 0.001, n = 5.