Figure 1
From: An arrayed CRISPR knockout screen identifies genetic regulators of GLUT1 expression
Establishment of a Cas9-expressing Caco-2 cell line with homogeneous GLUT1 expression. (A) Representative immunofluorescent image of mKate2 expression in clone C6 after sorting and outgrowth. Scale bar represented 200 µm. (B) Representative western blot assessment of Cas9 protein expression in wild-type Caco-2 cells versus clone C6. HSP60 was used as a loading control. Original blots are presented in Supplementary Fig. 5. (C) Assessment of Cas9 and GLUT1 expression after bulk sorting of C6-Caco-2 cells using an anti-GLUT1 antibody. Cas9 expression was assessed by western blot with HSP60 as a loading control. Original blots are presented in Supplementary Fig. 6. GLUT1 expression was assessed by immunostaining. Scale bars represent 50 µm.
