Figure 3
From: An arrayed CRISPR knockout screen identifies genetic regulators of GLUT1 expression
Arrayed screen workflow. (A) Schematic of screening procedure. Library plates were stamped and barcoded with a Bravo automated liquid handling platform, cells were distributed with a Multidrop Combi reagent dispenser for reverse transfection, medium was exchanged with an EL406 washer dispenser, and cells were fixed, stained, and imaged with an ImageXpress Micro XL automated imager. (B) Schematic layout for imaging in 96-well plates. Six images were taken from each well at the indicated locations (panel i) and control and experimental crRNAs were distributed within each plate as presented (panel ii). (C) Representative images taken from one 96-well plate.
