Figure 4
From: An arrayed CRISPR knockout screen identifies genetic regulators of GLUT1 expression
Primary screen outcomes. (A) Compiled CRISPR screening results for 8322 genes. Data represent the mean from n = 2 biological replicates per experimental condition and are binned by GLUT1 expression normalized to the non-targeting control. (B) Normalized GLUT1 expression from the library wells containing SLC2A1-targeting crRNA. Each data point is the mean of a single biological replicate calculated using six images taken from each well (n = 6 technical triplicates). Data are presented as mean ± standard deviation from the 2 biological replicates. Statistical significance was calculated using the Student’s unpaired t-test (*, p < 0.05). NT denotes non-targeting control. (C) Compiled datasets of hit distribution corresponding to biological function. Top panel (i) is hits that downregulated GLUT1 and bottom panel (ii) is hits that upregulated GLUT1. (D) Signaling pathways enriched for genes whose removal downregulated (i) or upregulated (ii) GLUT1 expression.
