Figure 1

Schematic diagram of steps undertaken in this study. A two-stage sampling design was followed for national-level surveillance of FMDV in India. At stage I villages are randomly selected from the considered states and in stage II animals are selected randomly from the selected villages (at stage I) for serological test through NSP-ELISA. After obtaining the sample-level DIVA test results data through NSP-ELISA, then such data was given input to the developed method and tool to compute various state and national-level measures for FMDV sero-surveillance.