Figure 5
From: Periostin facilitates ovarian cancer recurrence by enhancing cancer stemness
Exogenous periostin promotes lipid accumulation and is linked to lipid metabolism in the cancer microenvironment and in cancer cells. (A) POSTN-overexpressing 3T3-L1 preadipocytes exhibit enhanced lipid production. Imaging was performed in differentiated cells after Oil Red O staining at 10 × 10 magnification. The lipid drops stained with Oil Red O are indicated with an arrow. This test was independently repeated three times. (B) RNA sequencing data shows that the fatty acid synthesis gene (FASN) is more highly expressed in HEYA8 cells cultured under CMPOSTNhigh compared to CMCTL (six replicates; p < 0.001). This test was independently repeated three times. (C) FASN expression is higher in rOC than in matched pOC (p = 0.04). This test was independently repeated three times. (D) HEYA8 cells grown under CMPOSTNhigh conditions are more resistant to FASN inhibitor Cerulenin (0–60 µM) than those grown with CMCTL (p = 0.02). This test was independently repeated three times. (E) FASN inhibition combined with chemotherapy exhibits enhanced efficacy over that of either agent alone against HEYA8 cells under CMPOSTNhigh. Left, cells treated with cerulenin (CLN), paclitaxel (PTX), or CLN + PTX; right, cells treated with CLN, carboplatin (CRB), or CLN + CRB. This test was independently repeated three times.
