Table 3 Probes of the custom NSCLC gene fusion panel of Barretos Cancer Hospital in the Elements XT nCounter.
Elements XT nCounter panel genes and Housekeeper (HK) | |||||||
|---|---|---|---|---|---|---|---|
Probes for specific genes | |||||||
ALK-fusiona | ROS1-fusiona | RET-fusiona | MET∆ex14b | NTRK1-fusion | NTRK2-fusion | NTRK3-fusion | |
Probes 5′ and 3′ for imbalance fusion detection | ALK_ex1 | ROS1_ex1_(5’_UTR) | RET_ex1-2 | NTRK1_ex1 | NTRK2_ex1 | NTRK3_ex20b | |
ALK_ex5 | ROS1_ex18-19 | RET_ex2-3 | NTRK1_ex2-3-4 | NTRK2_ex3 | NTRK3_ex20 | ||
ALK_ex8-9 | ROS1_ex24 | RET_ex6-7 | NTRK1_ex5 | NTRK2_ex4 | NTRK3_ex19 | ||
ALK_ex18 | ROS1_ex29-30 | RET_ex11 | NTRK1_ex7 | NTRK2_ex5-6 | NTRK3_ex17 | ||
ALK_ex22-23 | ROS1_ex37 | RET_ex14-15 | NTRK1_ex14 | NTRK2_ex8 | NTRK3_ex9 | ||
ALK_ex26-27 | ROS1_ex40 | RET_ex15-16-17 | NTRK1_ex15 | NTRK2_ex18 | NTRK3_ex7 | ||
ALK_ex29 | ROS1_ex41/42 | RET_ex18 | NTRK1_ex17 | NTRK2_ex19 | NTRK3_ex4-5 | ||
ALK_ex29_(3′_UTR) | ROS1_ex43_(3′_UTR) | RET_ex19_(3′_UTR) | NTRK1_ex17b | NTRK2_ex20 | NTRK3_ex3 | ||
NTRK2_ex21 | |||||||
Probes for specific genes | EML4_ex13–ALK_ex20 | CD74_ex6–ROS1_ex32 | KIFB5_ex16–RET_ex12 | MET_ex13-14 | |||
EML4_ex20–ALK_ex20 | SDC4_ex2–ROS1_ex32 | KIFB5_ex22–RET_ex12 | MET_ex13-15 | ||||
EML4_ex6–ALK_ex20 | SLC34A2_ex13–ROS1_ex32 | KIFB5_ex23–RET_ex12 | |||||
EML4_ex18–ALK_ex20 | SLC34A2_ex4–ROS1_ex32 | CCDC6_ex1–RET_ex12 | |||||
KIF5B_ex24–ALK_ex20 | EZR_ex10–ROS1_ex34 | ||||||
KIF5B_ex17–ALK_ex20 | SDC4_ex4–ROS1_ex34 | ||||||
TFG_ex5–ALK_ex20 | GOPC_ex8–ROS1_ex35 | ||||||
GOPC_ex4–ROS1_ex36 | |||||||
LRIG3_ex16–ROS1_ex34 | |||||||
Housekeeper genes (HK) | SYMPK | HPRT1 | GAPDH | GUSB | OAZ1 | POLR2A | |
