Figure 5 | Scientific Reports

Figure 5

From: α-1,3-Glucanase from the gram-negative bacterium Flavobacterium sp. EK-14 hydrolyzes fungal cell wall α-1,3-glucan

Figure 5

Hydrolysis of the cell wall preparation of A. oryzae (A) and TLC analysis of reaction products from the cell wall preparation (B). The reaction mixture contained 2% cell wall preparation of A. oryzae, 50 mM potassium phosphate (pH 6.5), and 1.0 nmol/ml Agl-EK14. The mixture was incubated at 37 °C. (A) Each data point represents the mean ± standard deviation from triplicate experiments. (B) The 2–24h reaction products were spotted at 2 μl on TLC. TLC was performed as described in Methods. Glucose (Glc), nigerose (NG2), nigerotriose (NG3), and nigerotetraose (NG4) were used as markers. Lane M, markers; lane G, α-1,3-glucan hydrolytic products of Agl-EK14 after 24 h.

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