Figure 3
PRMT5 inhibition altered gene expression after 3 days of treatment. (A) HSJD-DIPG-007 cells were seeded as spheroids and treated with the LLY-283 GI40 the following day. Total cellular RNA was collected after a 0, 1, 2, 3, 5, 7 or 10 days treatment with LLY-283. RNA was collected and sequenced for 3 technical repeats for days 0 and 5, 2 technical repeats for day 7 and 1 technical repeat for days 1, 2 and 3. (B) The clustering of samples according to the 1st and 2nd principal components. Gene expression changes over time and diverges after 3 days along PC2 according to treatment group. (C) The relative expression of the 25 genes with the highest PC2 weighting (scaled by row). Column clustering shows the similarity between day 0 and vehicle samples, and the differential expression within later treated samples.
