Figure 4

(A) Gene expression profiling for histone modifiers, including histone methyltransferases, histone demethylases, histone acetyltransferases, and histone deacetylases. The yellow and dark blue represent higher and lower gene expression levels, respectively. (B) Immunofluorescence for MAP2+ and KAT2B + neurons in brain organoids with or without electromagnetized AuNPs. Scale bar = 20 µm. (C) Quantification of MAP2 and KAT2B-positive cells in each condition at 3 weeks. Data represent mean ± SEM. Student’s t-test, **P < 0.01; n = 5 per group. (D) Western blot analysis of KAT2B in brain organoids with or without electromagnetized AuNPs at different time points. Original blots are presented in Supplementary Fig. 4. Data represent mean ± SEM. Student’s t-test, *P < 0.05, **P < 0.01; n = 3 per group. (E) ChIP-PCR analysis showing the occupancy of KAT2B at the SLC2A1 promoter regions in brain organoids with or without electromagnetized AuNPs. Data represent mean ± SEM. one-way ANOVA, **P < 0.01; n = 3 per group. (F) Quantification of the binding of KAT2B at MT3 promoter regions in brain organoids with or without electromagnetized AuNPs. Data represent mean ± SEM. one-way ANOVA, **P < 0.01; n = 3 per group. Control-EMF brain organoid without AuNPs and EMF exposure, Control + EMF EMF-exposed brain organoid without AuNPs-EMF, AuNP AuNP-brain organoid without EMF exposure, AuNP + EMF AuNP-brain organoid with EMF exposure.